How To Calculate Rf Values
Retardation factor values are helpful in the identification in comparing unknown and known compounds. Using more solvents helps to obtain more rf values for more accurate identification.
The retention factor shows how far a substance moves relative to the solvent front.
How to calculate rf values. Rf values can be compared to help determine the identity of a compound. Compounds can have the same rf value for a singular solvent but not likely for multiple solvents. Retention factors are useful in comparing the results of one chromatogram to the results of another.
Rf a rf b rf c rf m components of m this problem has been solved. Rf values do not have units since it is a ration of distances. Notice that 1 the bigger the rf the further the spot moved and 2 that the rf should be the same for a component regardless of how far the solvent moves.
Learn how to calculate retention factors rf for thin layer chromatography tlc. If you re seeing this message it means we re having trouble loading external resources on our website. Because mixture solvents are often applied rf values are usually written as the following examples.
Chromatography allows you to separate mixtures in a lab. Calculate the rf values for a b and c in figure 16 9. Mobile phase mobility rf value stationary phase separation thin layer chromatography tlc what is tlc.
Tlc is a chromatography technique responsible for the separation of organic compounds on a mixture based on their relative mobility. The rf values for each of the components in the previous example can be calculated. Rf 0 66 60 ethanol if is given it is assumed that the mixture is in water hence 60 ethanol 40 water.
Show transcribed image text. Previous question next question. How to calculate rf values for tlc calculation of rf value.
What are the components of mixture m. The mixture is dissolved in the mobile phase and is separated by the mobile phase.
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